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Question #47763
The Question
A recombinant vector carrying a selectable marker under a strong constitutive promoter accidentally integrates into a human somatic cell genome via horizontal gene transfer, leading to unchecked cellular proliferation. Which of the following statements regarding this oncogenic transformation and the subsequent host immune surveillance is INCORRECT?
Options
- A.
The vector's integration may have caused insertional inactivation of a tumor suppressor gene, analogous to the inactivation of the lacZ gene during recombinant screening.
- B.
The strong constitutive promoter of the selectable marker could have activated an adjacent cellular proto-oncogene (c-onc) into an oncogene.
- C.
The primary host defense mechanism to eliminate this transformed cell relies on the humoral immune response, specifically B-lymphocytes producing antibodies against the selectable marker.
- D.
Natural Killer (NK) cells and Cytotoxic T-lymphocytes form the core immune surveillance system to detect and induce apoptosis in such neoplastically transformed cells.
Check Your Answer
Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Evolutionary_Exception
Tackle problems where standard rules evolve or change based on specific environmental constraints.
Concepts Behind This Question
PCR Primer Design|Human Genome Repetitive DNA|DNA Polymerase Directionality
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: This question synthesizes 'insertional inactivation' from Biotechnology with 'oncogenesis' and 'immune surveillance' from Human Health and Disease.
The trap relies on Functional Confusion: students often assume that because a selectable marker produces a 'foreign protein', the Humoral immune system (antibodies) will be the primary defense.
However, for intracellular anomalies and neoplastic transformations, Cell-Mediated Immunity is the definitive physiological response.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biotechnology : Principles and Processes - Class 12 |
| Concept | PCR Primer Design |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |