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Question #47613

Difficulty: 8/10 | 🟢 Free

The Question

During a laboratory experiment, isolated intact chloroplasts of a typical C3C_3 plant are subjected to extreme heat stress (50∘C50^\circ C). Despite maintaining an optimal stromal concentration of CO2CO_2, ATP, and NADPH, the synthesis of 3-phosphoglycerate (3-PGA) completely ceases. What is the primary physiological reason for this observation?

Options

  • A.

    The affinity of RuBisCO for O2O_2 significantly exceeds that for CO2CO_2 at this temperature, leading to exclusive photorespiration.

  • B.

    The thermal disruption of non-covalent bonds in RuBisCO alters its tertiary structure, abolishing its catalytic active site.

  • C.

    The high temperature uncouples the proton gradient across the thylakoid membrane, preventing the utilization of the proton motive force.

  • D.

    The accumulation of NADPH causes severe allosteric feedback inhibition of the reduction phase enzymes in the Calvin cycle.

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Correct Answer

Step-by-Step Solution

Explanation

How the Examiner Thinks

Regulatory_Failure

Understand how NOT, EXCEPT, and logical constraints are used to manipulate exam results.

Concepts Behind This Question

C3 Cycle Stoichiometry|Protein Denaturation Specificity|Rubisco

Why This Question Is Tricky

Common Mistakes

Conceptual Pivot: Distinguishing between a kinetic shift in enzyme affinity (oxygenase vs. carboxylase activity) and the absolute structural collapse of the enzyme (denaturation).

Students reflexively associate 'high temperature in C3 plants' solely with 'increased photorespiration' (Option A).

They fail to realize that photorespiration still yields 3-PGA, and that extreme heat causes actual protein denaturation, breaking the stoichiometry of the Reductive Pentose Phosphate Cycle entirely.

Quick Facts About This Question

Question Information

SubjectBiology
ChapterPhotosynthesis in Higher Plants - Class 11
ConceptC3 Cycle Stoichiometry
Difficulty8
Cognitive Load5
Target Solve Time120 sec
AccessFree