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Question #46548
The Question
To arrest metastasis in a highly invasive carcinoma, researchers utilize RNA interference (RNAi) to silence the mRNA of Matrix Metalloproteinases (MMPs), enzymes that degrade the basement membrane. However, clinical application reveals severe toxicity due to the degradation of unintended, essential homeostatic mRNAs. Which of the following best explains the molecular basis of this specific limitation in RNAi therapeutics?
Options
- A.
The synthetic dsRNA directly binds to the promoter regions of non-target genes, inhibiting their transcription.
- B.
The siRNA-loaded RNA-induced silencing complex (RISC) cleaves non-target mRNAs that share partial sequence homology with the MMP mRNA.
- C.
The introduced siRNA competitively inhibits the Dicer enzyme, preventing the translation of all endogenous cellular mRNAs.
- D.
The targeted MMP mRNA acts as a silencing inhibitor by degrading the Argonaute protein within the RISC complex.
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Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Regulatory_Failure
Understand how NOT, EXCEPT, and logical constraints are used to manipulate exam results.
Concepts Behind This Question
mRNA_Translation|Post-transcriptional_Regulation|Invasive_Enzymes
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: Students often interpret NCERT's description of RNAi silencing a 'specific mRNA' as an absolutely flawless lock-and-key mechanism.
The trap tests the understanding of 'off-target effects' due to partial sequence homology, a critical limitation in translating biotechnology principles into clinical therapeutics.
It also tests the fundamental location and timing of RNAi (post-transcriptional vs transcriptional).
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Human Health and Disease - Class 12 |
| Concept | Metastasis |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |