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Question #45742
The Question
An experimental mammalian model is subjected to RNA interference (RNAi) targeting the mRNA of the specific carrier proteins responsible for the active reabsorption of glucose in the Proximal Convoluted Tubule (PCT). Assuming the Glomerular Filtration Rate (GFR) remains unaltered, which of the following statements correctly describes the molecular mechanism and its immediate physiological consequence?
Options
- A.
The targeted gene undergoes a frameshift mutation, permanently halting secondary active transport and causing compensatory glucose reabsorption in the Distal Convoluted Tubule (DCT).
- B.
The complementary dsRNA prevents the transcription of the carrier protein gene, leading to glycosuria and a significant decrease in the volume of filtrate entering the descending limb of Henle's loop.
- C.
The specific mRNA is silenced post-transcriptionally, abolishing glucose reabsorption which subsequently induces osmotic diuresis and increases the volume of tubular fluid.
- D.
The spliceosome complex is inhibited from processing the pre-mRNA, causing the brush border epithelium to lose its microvilli and resulting in severe oliguria.
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Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Regulatory_Failure
Understand how NOT, EXCEPT, and logical constraints are used to manipulate exam results.
Concepts Behind This Question
Proximal Convoluted Tubule Selectivity|Spliceosome Mechanics|RNA Interference (RNAi) Mechanism
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: This question tests the integration of Molecular Biology (Biotechnology applications) with Human Physiology (Excretory products).
The primary trap is confusing the level at which RNAi operates (post-transcriptional translation block vs. genomic mutation/transcriptional block).
The secondary trap requires students to apply the concept of osmolarity to renal physiology: unabsorbed solutes (glucose) hold water in the lumen, leading to osmotic diuresis rather than a decrease in filtrate volume.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Excretory Products and Their Elimination - Class 11 |
| Concept | Proximal Convoluted Tubule Selectivity |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |