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Question #45306
The Question
A child inherits a genetic disorder caused by an unequal exchange of homologous chromosome segments during gametogenesis in the father. A physician attempts to diagnose the exact genomic structural variant in the child using ELISA but is unsuccessful. Which of the following correctly identifies the meiotic stage of the original error and the reason for the diagnostic failure?
Options
- A.
Zygotene; ELISA detects only the phenotypic protein expression, not the primary DNA sequence alteration.
- B.
Pachytene; ELISA is based on antigen-antibody interactions and cannot detect DNA-level structural variations.
- C.
Pachytene; ELISA can only detect somatic DNA mutations and is incapable of identifying heritable germline variants.
- D.
Diplotene; ELISA detects RNA transcripts rather than the genomic DNA alterations caused by recombinase errors.
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Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Regulatory_Failure
Understand how NOT, EXCEPT, and logical constraints are used to manipulate exam results.
Concepts Behind This Question
Pachytene|Molecular Diagnosis by ELISA|Germline_Mutation
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: Students often memorize that 'ELISA is a molecular diagnostic tool' (NCERT Biotechnology) but fail to distinguish its target (proteins/antibodies) from PCR or Southern blotting (DNA).
Additionally, it tests the precise meiotic sub-stage (Pachytene) where recombinase-mediated crossing over occurs, linking chromosomal aberrations to germline heritability.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Cell Cycle and Cell Division - Class 11 |
| Concept | Pachytene Crossing Over |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |