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Question #45162
The Question
During an experimental somatic gene therapy, a viral vector delivering an RNAi construct accidentally integrates into the germline of a mammalian model. This triggers stable DNA methylation and histone modification at an essential developmental gene locus. Which of the following is the most likely consequence regarding the inheritance and expression of this trait?
Options
- A.
It will follow standard Mendelian segregation ratios as the RNAi vector induces a frameshift mutation in the target gene.
- B.
It will exhibit non-Mendelian, transgenerational epigenetic inheritance without altering the primary nucleotide sequence of the target gene.
- C.
The silencing will be strictly maternal because the Dicer-RISC complex operates exclusively within the mitochondrial matrix.
- D.
The trait will not be inherited by the F1 progeny because RNA interference is strictly a transient, post-translational defense mechanism.
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Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Regulatory_Failure
Understand how NOT, EXCEPT, and logical constraints are used to manipulate exam results.
Concepts Behind This Question
RNAi|Germline Transmission|Vector delivery
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: Differentiating between structural DNA mutations (which follow Mendelian genetics) and epigenetic chromatin modifications (which exhibit non-Mendelian inheritance).
Common Misconception: Students often misclassify RNAi as a post-translational process or assume that any heritable, long-term gene silencing must involve a direct alteration of the DNA nucleotide sequence.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biotechnology and Its Applications - Class 12 |
| Concept | Gene Therapy Germline vs Somatic |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |