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Question #45120
The Question
Baculoviruses (genus Nucleopolyhedrovirus) are utilized as narrow-spectrum insecticidal agents because they have no negative impacts on non-target organisms, including mammals. If a researcher uses the biolistic method (gene gun) to directly deliver a recombinant baculovirus DNA vector into the nucleus of a mammalian cell, which of the following best explains why the vector still fails to establish a systemic infection?
Options
- A.
The mammalian cell lacks the specific cell-surface receptors required for the baculovirus to enter and initiate the infection cycle.
- B.
The mammalian intracellular machinery lacks the compatible transcription factors required to recognize and activate the viral promoters.
- C.
The viral DNA is immediately degraded by mammalian restriction endonucleases present in the cytoplasm.
- D.
The biolistic delivery method triggers an immediate hypersensitive response, leading to mammalian cell apoptosis.
Check Your Answer
Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Regulatory_Failure
Understand how NOT, EXCEPT, and logical constraints are used to manipulate exam results.
Concepts Behind This Question
Biolistics/Gene Gun Physics|Baculovirus Host Specificity
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: Students often memorize that 'viruses are host-specific due to cell-surface receptors' (NCERT Human Health/Microbes).
The trap is failing to realize that if this physical barrier is bypassed via biotechnology (biolistics/microinjection), the secondary, definitive biological checkpoint is the compatibility of the host's intracellular machinery (transcription factors, promoters) with the viral genome.
Option A is the primary trap designed to catch students who do not apply the 'If/Then' logic of the biolistic bypass.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biotechnology : Principles and Processes - Class 12 |
| Concept | Biolistics/Gene Gun Physics |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |