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Question #135243
The Question
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): In a continuous culture bioreactor, a miscalibrated thermal control system that causes mild heat shock significantly reduces the yield of the target recombinant protein in E. coli. Reason (R): Thermal stress induces the transcription of heat-shock genes, diverting cellular ATP towards the synthesis of chaperone proteins rather than biomass and target protein accumulation.
In the light of the above statements, choose the most appropriate answer from the options given below:
Options
- A.
Both (A) and (R) are correct and (R) is the correct explanation of (A)
- B.
Both (A) and (R) are correct but (R) is not the correct explanation of (A)
- C.
(A) is correct but (R) is not correct
- D.
(A) is not correct but (R) is correct
Check Your Answer
Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Comparative_Analysis
Sharpen your ability to evaluate multiple scenarios and distinguish between competing potential answers.
Concepts Behind This Question
Bioreactor Geometry|Heat Shock Thermal Gradient|Optimal Foraging Theory
Why This Question Is Tricky
Examiner Levers
Logical Assertion
Tests the causal relationship between two true statements.
Difficulty Boost : +4Common Mistakes
Conceptual Pivot: Students often memorize that bioreactors require 'optimal temperature' (NCERT Biotechnology) but fail to connect the physiological consequence of temperature deviation to the metabolic energy budget.
The trap is failing to recognize that mild heat shock doesn't just passively 'slow down' enzymes, but actively triggers a competing, ATP-draining genetic response (chaperone synthesis) that directly cannibalizes the energy meant for recombinant protein yield.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biotechnology : Principles and Processes - Class 12 |
| Concept | Bioreactor Geometry |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |