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Question #133136
The Question
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): The accidental integration of a cloning vector's selectable marker cassette into a human somatic cell genome can potentially induce neoplastic transformation. Reason (R): Strong constitutive promoters driving the selectable marker can cause insertional mutagenesis, leading to the activation of cellular proto-oncogenes and overwhelming host immune surveillance. In the light of the above statements, choose the most appropriate answer from the options given below:
Options
- A.
Both (A) and (R) are correct and (R) is the correct explanation of (A)
- B.
Both (A) and (R) are correct but (R) is not the correct explanation of (A)
- C.
(A) is correct but (R) is not correct
- D.
(A) is not correct but (R) is correct
Check Your Answer
Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Comparative_Analysis
Sharpen your ability to evaluate multiple scenarios and distinguish between competing potential answers.
Concepts Behind This Question
Selectable Marker vs Cloning Site|Oncogenes vs Proto-oncogenes|Metastasis
Why This Question Is Tricky
Examiner Levers
Logical Assertion
Tests the causal relationship between two true statements.
Difficulty Boost : +4Common Mistakes
Conceptual Pivot: Students often restrict the concept of 'insertional mutagenesis' strictly to retroviruses (as per NCERT Human Health chapter).
This question requires synthesizing Biotechnology and Cancer Biology, realizing that ANY strong promoter (like those in selectable marker cassettes) integrating near a proto-oncogene can act as an insertional mutagen and bypass immune surveillance.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biotechnology : Principles and Processes - Class 12 |
| Concept | Selectable Marker vs Cloning Site |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |