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Question #133113
The Question
During the large-scale production of a recombinant therapeutic protein using a lac promoter-driven E. coli system, excessive and premature addition of the inducer causes severe 'metabolic burden' on the host cells. Which of the following statements regarding the subsequent downstream processing of this batch is INCORRECT?
Options
- A.
The biological purity of the final product is significantly compromised due to high levels of host cell protein (HCP) contamination.
- B.
Downstream processing becomes highly efficient because premature cell lysis naturally releases the intracellular product, bypassing the need for mechanical cell disruption.
- C.
The overall yield of the biologically active drug decreases due to the release of endogenous host proteases into the culture medium.
- D.
The separation phase is severely hindered by the increased viscosity of the culture broth caused by the release of host genomic DNA.
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Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Regulatory_Failure
Understand how NOT, EXCEPT, and logical constraints are used to manipulate exam results.
Concepts Behind This Question
Inducer/IPTG|Downstream Processing|E. coli
Why This Question Is Tricky
Examiner Levers
Pattern Traps
Forces student to avoid "obvious" but wrong patterns or sequences.
Difficulty Boost : +2Common Mistakes
Conceptual Pivot: Students often fall for the 'Common Half-Truth' trap.
They know that intracellular proteins require cell lysis for extraction.
Therefore, they incorrectly assume that premature lysis in the bioreactor is beneficial because it 'saves a step' in downstream processing.
This fails to distinguish between controlled mechanical disruption during the purification phase and uncontrolled, stress-induced lysis during the growth phase, which ruins the batch via protease degradation and extreme viscosity.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biotechnology : Principles and Processes - Class 12 |
| Concept | Downstream Processing |
| Difficulty | 5 |
| Cognitive Load | 4 |
| Target Solve Time | 100 sec |
| Access | Free |