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Question #132921
The Question
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): The genetic integrity of germplasm maintained via ex-situ conservation can be severely compromised if molecular identification relies on PCR amplification with low primer annealing stringency. Reason (R): Low annealing temperature reduces the specificity of hydrogen bonding between primers and the template DNA, leading to the amplification of non-target sequences and the potential misidentification of genetic mosaics as pure lines.
In the light of the above statements, choose the most appropriate answer from the options given below:
Options
- A.
Both (A) and (R) are correct and (R) is the correct explanation of (A)
- B.
Both (A) and (R) are correct but (R) is not the correct explanation of (A)
- C.
(A) is correct but (R) is not correct
- D.
(A) is not correct but (R) is correct
Check Your Answer
Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Comparative_Analysis
Sharpen your ability to evaluate multiple scenarios and distinguish between competing potential answers.
Concepts Behind This Question
Cryopreservation|Downstream Processing|Germplasm
Why This Question Is Tricky
Examiner Levers
Logical Assertion
Tests the causal relationship between two true statements.
Difficulty Boost : +4Common Mistakes
Conceptual Pivot: Students often memorize the three steps of PCR (Denaturation, Annealing, Extension) without understanding the thermodynamic implications of the annealing temperature.
The core misconception is failing to link molecular biotechnology (primer stringency and PCR fidelity) with macroscopic ecological applications (maintaining germplasm purity in ex-situ conservation).
Understanding that 'low stringency' equates to 'high error rate/non-specific binding' is crucial to solving this.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biotechnology : Principles and Processes - Class 12 |
| Concept | Downstream Processing |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |